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The following qRT-PCR conditions were used: 40 cycles of 94 °C for 15 s, annealing temperatures as indicated in Table (1) for 30 s, and extension temperature at 72 °C for 20 s were performed after 95 °C for 4 min

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The anti-apoptotic effects of GPS in neonatal rat hippocampal neurons following oxygen–glucose deprivation and reperfusion injury was tested by Wang et al