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coli mutants Bacterial colonies from individual bacterial mutants were streaked on LB agar plates, grown at 37 °C, and genotyped by PCR using the kanamycin-cassette-specific primers (forward primer 5’-CGGTGCCCTGAATGAACTGC-3’, reverse primer 5’-CAGTCATAGCCGAATAGCCT-3’) and genomic primers for individual bacterial mutants, which were designed at starting 100-300 bases upstream of start-codon (forward primer) or downstream of stop codon (reverse primer)
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This observation is interesting, as NFκB activation is considered antiapoptotic and pro-survival, in part, due to its role in augmenting the expression of IAPs, which attenuate caspase activation