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However, using this knowledge we expect the formation of a hydroxyl group adjacent to the disulphide bond (as seen in product 7) as well as a peptide break after the oxidation of peroxide 5

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Aliquots of 25 μL of the reaction mixtures were plated onto chocolate agar in duplicates at the beginning of the assay (t 0 ) and again after incubation at 37 °C for 30 min (t 30 )