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Each reaction mixture was prepared using 10 μl QuantiFast SYBR Green PCR master mix, 4 μl of cDNA template with 1 μM of each primer in a total reaction volume of 20 μl

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Doses Of Vitamin C For Scurvy The British National Formulary (the “prescriber’s bible”) currently recommends at least 250 mg of vitamin C daily (in divided doses) to treat scurvy